Gene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut in Escherichia coli with clinical evaluation of antiwrinkle efficacy
This study describes the design, bacterial expression, and functional analysis of recombinant conotoxins TIIIA and TIIIAlaMut. Their bioactivity was confirmed on human Nav1.4 channels. The recombinant toxins, including the form extracted from the cream, showed effects comparable to a synthetic standard. Application tests demonstrated the conotoxin's potential in cosmeceuticals, particularly in reducing periocular wrinkles and improving skin texture and tone.
Authors
- Jolien De Waele (ORCID: https://orcid.org/0000-0003-0674-4521)
- Agata Stefanek
- Tomasz Ciach (ORCID: https://orcid.org/0000-0002-8726-8944)
- Anna Mazurkiewicz-Pisarek (ORCID: https://orcid.org/0000-0001-9097-8894)
- Magdalena Janczewska
- Alina Mazurkiewicz
- Przemysław Styczeń
- Diana Mikiewicz
- Agnieszka Lew-Mirska
- Frank Bosmans
Institutions
- Warsaw University of Technology (PL)
- Vrije Universiteit Brussel (BE)
- Ghent University (BE)
- Aesthetic Surgery Center (US)
- Deutsches Historisches Institut Warschau (PL)
Publication Details
- Journal
- BioTechnologia
- Published
- 2025-12-23
- DOI
- https://doi.org/10.5114/bta/214377
- Primary Topic
- Nicotinic Acetylcholine Receptors Study
- Type
- article
- Field-Weighted Citation Impact
- 0.00