Impaired Aβ Clearance Mechanisms at the Inner Blood‐Retina Barrier in Alzheimer's Disease: Insights from Ex Vivo Retinal Imaging
The imaging plane (wholemount vs. cross-section) may influence outcomes in AD pathogenesis studies. Wholemount analysis revealed glymphatic clearance and microglial phagocytosis as compensatory mechanisms for mitigating Aβ accumulation, with peripheral macrophage-like monocytes contributing to SAβO clearance in control neuroretinas. These mechanisms were largely disrupted in AD donors. Ex vivo 3D retinal imaging, applied here for the first time to study clearance at the iBRB, provides novel insights into retinal and BBB analog processes in AD.
Authors
- Joanne A. Matsubara (ORCID: https://orcid.org/0000-0002-6236-9191)
- Matthew E. Campbell (ORCID: https://orcid.org/0000-0003-3325-240X)
- Justin R. Haynes
- Jeanne Xi
- Amir Hosseini
- Benjamin W. Spiller (ORCID: https://orcid.org/0000-0001-5397-1028)
- Ian R. Mackenzie (ORCID: https://orcid.org/0000-0003-3875-2972)
- Wellington Pham (ORCID: https://orcid.org/0000-0002-8408-7611)
- Brian E. Wadzinski (ORCID: https://orcid.org/0000-0002-2980-3697)
- Shivani Tejpal
- Veronica Hirsch‐Reinshagen
- Printha Wijesinghe
- Ging‐Yuek Robin Hsiung
Institutions
- Vancouver Coastal Health (CA)
- University of British Columbia (CA)
- Vanderbilt University Medical Center (US)
Publication Details
- Journal
- Alzheimer s & Dementia
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1002/alz70855_104480
- Primary Topic
- Alzheimer's disease research and treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00