Mass spectrometry imaging of Alzheimer's disease vulnerable and resilient neocortical areas in postmortem formalin‐fixed paraffin‐embedded and fresh frozen human brains

This study demonstrates the viability and limitations of TMA FFPE compared with frozen tissues to map brain molecules by MSI. Despite frozen tissues yielding higher metabolite recovery, FFPE tissues are widely available in brain banks and more easily integrated with histology and immunofluorescence techniques. Thus, this spatial MSI is a valuable tool to evaluate molecular changes more broadly across brain regions in AD, offering insights into disease progression and correlated biomarkers.