Single cell transcriptomic profiling uncovers shared inflammatory pathways and immune dysregulation underlying the asthma to lung cancer transition

Through investigating immune cell spatiotemporal behaviors and key gene activities, our research illuminates the intricate immune architecture within lung cancer microenvironments. Personalized immunotherapy development will be guided by these insights, which clarify how specific immune cells function while exposing prospective therapeutic intervention points. Additionally, qRT-PCR validation in lung cancer cell lines confirmed the differential expression of CXCL12, CXCR4, and PD-L1, reinforcing the robustness and translational relevance of our single-cell findings.