A Comparison of Flow Cytometry-based versus ImmunoSpot- or Supernatant-based Detection of SARS-CoV-2 Spike-specific Memory B Cells in Peripheral Blood
The study demonstrates that all three assays are suited for the detection of specific B<sub>mem</sub> in antigen-primed individuals when such B<sub>mem</sub> occur in the mid- to high-frequency range, and that they broadly concur. Strengths and weaknesses of the three test systems are discussed.
Authors
- Sharon Cookson (ORCID: https://orcid.org/0000-0001-9476-6741)
- Greg A. Kirchenbaum (ORCID: https://orcid.org/0000-0001-6463-7623)
- Stephen Todryk (ORCID: https://orcid.org/0000-0003-4566-0793)
- Paul Lehmann
- Georgia Stylianou (ORCID: https://orcid.org/0009-0000-9062-8157)
- Justin T. Nassif
Institutions
- Northumbria University (GB)
- Shaker Heights Public Library (US)
- Newcastle University (GB)
Publication Details
- Journal
- Vaccines
- Published
- 2025-12-24
- DOI
- https://doi.org/10.3390/vaccines14010020
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00