Response Mechanism of Lactobacillus plantarum under Simulated Digestion Based on Proteomics and Metabolomics Analysis

Proteomics and a metabolomics strategy were used to investigate the probiotic properties of Lactobacillus plantarum BW2013 under simulated in vitro digestion. Thirty seven significant differential proteins including 6-phosphogluconate dehydrogenase, pyruvate kinase, F0F1 ATP synthase, ABC-type transporters, ribosomal and universal stress proteins and 40 significant differential metabolites, such as indole-3-lactic acid, Pro, trans-cinnamic acid, betaine, citric acid, D-fructose, and γ-aminobutyric acid were identified and characterized. The association analysis indicated that most upregulated proteins and metabolites are involved primarily in stress response, regulation of intestinal flora and accumulation of beneficial substances relating to carbohydrate and energy metabolic pathways, amino acid metabolism pathways, nucleotide metabolism pathways, overall/universal stress response. The results provide new insights into the response mechanism of L. plantarum BW2013 under the simulated digestion conditions, facilitating the exploitation of its potential application in probiotic products.