Cineole alters liver fat metabolism and enriches the fatty acid content of eggs in laying hens

This study evaluated 0.1%, 0.2%, and 0.3% dietary 1,8-cineole on laying hens' egg quality (focusing on fatty acid composition and cholesterol), using GC-MS, biochemical tests, and qPCR. 1,8-cineole had no adverse effects on egg thickness, albumin ratio, feed consumption, body weight, or egg production, but significantly increased egg weight and yolk saturated, monounsaturated, and polyunsaturated fatty acids. It upregulated key fat metabolism genes and reduced abdominal fat, adiponectin, LDL cholesterol, ACC, and FASN. These findings indicate 1,8-cineole can modify egg quality and lipid metabolism, potentially producing healthier and more nutritious eggs.