A novel inducible CRISPRi tool, CRISPRi‐Cre, to study neuron‐specific phenotypes in iPSC‐derived neuron models of Alzheimer's Disease
Here, we developed a robust Cre-inducible CRISPRi system that enables post-mitotic gene knockdown in iPSC-derived neurons. Our CRISPRi screens identify neuron-specific hits, demonstrating the utility of our tool to help uncover disease-relevant mechanisms, modifiers, and potential therapeutic targets in relevant cell types.
Authors
- Erika Lara
- Matthew P. Nelson (ORCID: https://orcid.org/0000-0001-7819-0922)
- Liz Calzada
- Mark Cookson
- Cory A. Weller (ORCID: https://orcid.org/0000-0001-6965-5599)
- Samuel S. Neuman
- Caroline B. Pantazis
- Marianita Santiana (ORCID: https://orcid.org/0000-0002-5133-847X)
- Daniel M. Ramos (ORCID: https://orcid.org/0000-0002-4041-5273)
- Andy Qi
- M. A. Nalls
- AB Singleton
- Michael Ward
- Sanjana Krishna
- William C Skarnes
Institutions
- National Institutes of Health (US)
- Data Tecnica International (United States) (US)
- National Institute of Neurological Disorders and Stroke (US)
- Jackson Laboratory (US)
- Institute on Aging (US)
- National Institute on Aging (US)
Publication Details
- Journal
- Alzheimer s & Dementia
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1002/alz70855_097245
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00