Negatively‐correlated tRF5 expressions with mRNA and protein expressions in iPSC‐derived microglia cells in Alzheimer’s disease

Analysis of iPSC-derived microglia revealed significant in vitro tRF5 expression differences and related mRNA/protein targets. HLA-DR and WDR1 proteins were identified as downregulated and negatively correlated with multiple tRFs (correlation p < 0.05) while their respective mRNA UTRs and tRFs had strong interaction sites, suggesting tRF5 regulation with these genes. We also noted downregulated HLA-DR and AIF1, suggesting roles of brain microenvironments in disease pathology.