Gap-Free T2T assemblies of Micropterus salmoides identify a Y-Linked SV hotspot underlying sexual dimorphism

This study provides the complete T2T genomes for LMB, correcting structural errors in prior assemblies and resolving repetitive regions critical for understanding sexual dimorphism. The SVER-Y region, enriched in male-specific SVs and TEs, offers novel insights into early Y chromosome differentiation. These resources establish a foundation for deciphering sex determination mechanisms and optimizing marker-assisted breeding strategies in LMB aquaculture.