Comparative study of structural properties of polymeric matrixes

Objective. Comparative analysis of the pore diameter and fiber thickness of polymer matrices with an assessment of the possibility of their colonization by cell cultures. Material and methods. Porous collagen matrices based on marine collagen (MC GA), (MC GLIO), developed on the basis of KSMU and KSU (Kursk) were used as the study material. Electron microscopy of the samples was carried out with measurements of the pore diameter and fiber thickness. Results. As a result of the study of the pore diameter, the highest value was found in MC KRS 555.2 [508.7; 602.3] (SEM 19.61) μm, in the MC GLIO group the diameter was 376.1 [204.7; 676.6] (SEM 66.59) µm and in the MC GA group 102.2 [90; 115.4] (SEM 4.445) µm. Statistically significant differences were found between the MC GA and MC GLIO groups (p=0.0032) and between the MC GA and BSE MC groups (p<0.0001). The greatest fiber thickness was found in the MC GA samples 42.25 [35.65; 48.93] (SEM 2.704) µm, in the BSE group 21.2 [17.88; 25.08] (SEM 1.101) µm and in the MC GLIO group 22.1 [17.38; 27.08] (SEM 1.683) µm. Statistically significant differences were found between MC GA and MC GLIO (p=0.0008) and between MC GA and MC BSE (p=0.0002). Conclusion. Thus, the pore diameter of MC BSE is 1.5 times larger than that of MC GLIO and 5.4 times higher than that of MC GA (p≤0.05). The thickness of the matrix fibers of MC GA is 2 times larger than that of BSE and 1.9 times higher than that of MC GLIO (p≤0.05). A comprehensive assessment of these parameters allows us to conclude that the choice of MC GA as a cell culture carrier is the most rational.