Surface Plasmon Resonance Sensors Based on the Molecularly Imprinted Technique for Simvastatin Detection

In this study, surface plasmon resonance (SPR)-based sensors were developed to determine simvastatin (SIM) with phosphate buffer (pH 7.4) using the molecular imprinting technique (MIT). SIM imprinted (MIP) and nonimprinted (NIP) poly-(2-hydroxyethyl methacrylate-<i>N</i>-methacryloyl-l-tryptophan methyl ester) polymeric films were synthesized onto the surface of the SPR chips to obtain kinetic parameters. The characterization of the MIP and NIP sensors was determined by contact angle and atomic force microscopy measurements. The range of linearity was measured as 0.001-1.0 mg/mL for SIM imprinted polymeric film-based SPR sensors. The selectivity of SPR sensors for competitive adsorption of atorvastatin and rosuvastatin was also investigated. After optimizing the experimental studies for SIM determination, SIM determination was also performed in artificial plasma solutions, and the recoveries were calculated to be approximately 99%. The findings indicate that SIM imprinted SPR sensors demonstrate exceptional sensitivity and selectivity along with a remarkably low detection limit for precise target identification.