Temporal Tracking of Metabolomic Shifts in In Vitro-Cultivated Kiwano Plants: A GC-MS, LC-HRMS-MS, and In Silico Candida spp. Protein and Enzyme Study

Cucumis metuliferus E. Mey or kiwano/African horned melon is a good source of bioactive compounds of various pharmacological and industrial importance. This study investigated metabolomic shifts in in vitro cultivated kiwano plants over ten weeks of maturity time through GC-MS and LC-HRMS-MS untargeted analysis of volatile and non-volatile metabolites. Furthermore, in silico screening of the highly abundant volatile compounds from each sample was performed against three different proteins and enzymes of Candida spp. These results obtained from GC-MS and LC-HRMS-MS analysis highlight the potential of in vitro culture for enhancing the biosynthetic potential of C. metuliferus for sustainable and controlled production of target metabolites. Furthermore, this work also highlights the potential inhibitory properties of abundant volatile compounds in each stage of maturation period of C. metuliferus, providing a platform for further exploration of the therapeutic applications of C. metuliferus metabolites against Candida spp.