Effect of Temperature on Fertilization and Hatchling Rate of Clarias batrachus induced with Ovatide

Abstract: Research was conducted on the induced breeding of the Asiatic catfish (Clarias batrachus), a species of high medicinal and economic value that is becoming increasingly scarce in the wild. The primary objective was to optimize fertilization and hatching rates by testing various dosages of the synthetic hormone Ovatide across different thermal environments, while also identifying the ideal temperature for hatchling survival. The study involved injecting female broodstock (weighing 132–144g) with Ovatide doses ranging from 0.5 to 2.5 ml/kg of body weight. These trials were carried out at five specific temperatures: 26°C, 27°C, 28°C, 29°C, and 30°C. Optimal Dosage: 1 ml/kg of body weight. Optimal Temperature: 28°C. Peak Results: This combination yielded the highest success rates, achieving 73.52% fertilization and 78.98% hatching. Keywords: Clarias batrachus, ovatide, inducing agents, fertilization, temperature. Title: Effect of Temperature on Fertilization and Hatchling Rate of Clarias batrachus induced with Ovatide Author: Dr. Tushar G. Deshmukh International Journal of Life Sciences Research ISSN 2348-313X (Print), ISSN 2348-3148 (online) Vol. 13, Issue 4, October 2025 - December 2025 Page No: 55-58 Research Publish Journals Website: www.researchpublish.com Published Date: 22-December-2025 DOI: https://doi.org/10.5281/zenodo.18015674 Paper Download Link (Source) https://www.researchpublish.com/upload/book/Effect%20of%20Temperature%20on%20Fertilization-22122025-1.pdf