Biocompatibility Evaluation of Novel Experimental Titanium Alloys for Dental Implants

<b>Background/Objectives</b>: The purpose of this study was to assess the in vitro biocompatibility and corrosion resistance of five titanium alloys that have been recently developed for dental implant applications, whose compositions were designed to align with current approaches in the development of novel biomaterials. Priority was given to limiting the harmfulness associated with specific chemical elements present in common conventional alloys and increasing corrosion resistance to improve the biomaterial-tissue cellular interaction. <b>Methods</b>: For this purpose, five types of titanium alloys with original chemical compositions (Ti1-Ti5) were developed. The electrochemical behavior of the alloys was analyzed by evaluating the corrosion resistance in environments that simulate the oral environment, as well as the cellular behavior, by evaluating the viability, growth, and proliferation of human cells on osteoblasts and gingival fibroblasts. Detailed analysis of the chemical composition by scanning electron microscope (SEM/EDS) methods was used. The corrosion rate of the alloys in artificial saliva was tested using the polarization resistance technique (Tafel). Human osteoblasts (hFOB cell line) and human gingival fibroblasts (hFIB-G cell line) were used to measure biocompatibility in vitro. <b>Results</b>: The Ti5 alloy demonstrated the highest cell viability and the lowest corrosion rate (0.114 μm/year) among all tested compositions, with the Ti3 alloy containing Mo and Zr following closely behind. The Ti2 alloy exhibited reduced biocompatibility because of the inclusion of Ni and Fe in its composition. <b>Conclusions:</b> Taken together, the results of this study provide useful information on the basic characteristics of titanium alloys with original chemical compositions. The titanium alloys were analyzed in comparison with common conventional alloys (Cp-Ti and Ti6Al4V) as well as alloys such as Ti-Zr, Ti-Nb, and Ti-Nb-Zr-Ta, which are considered to be viable alternatives to conventional materials for making dental implants.