Antibiotic Resistance Patterns of Bacteria Isolated from Canine Skin and Ear Infections in Serbia

<b>Background</b>: Canine skin and ear infections are common in small-animal practice and increasingly complicated by multidrug resistance (MDR), yet data from Serbia are limited. This study aimed to describe the bacterial etiology and antimicrobial resistance patterns in canine otitis externa and pyoderma. <b>Methods</b>: We retrospectively reviewed laboratory records from the Clinical Bacteriology and Mycology Laboratory, Faculty of Veterinary Medicine, University of Belgrade (January 2017-August 2024). A total of 422 non-invasive swabs from clinically ill dogs were included (ears: <i>n</i> = 210; skin: <i>n</i> = 212). Bacterial identification used conventional methods and commercial systems, and disk-diffusion susceptibility testing followed CLSI/EUCAST guidance. Methicillin resistance in staphylococci was assessed by cefoxitin/oxacillin screening; MRSA was confirmed by PCR and PBP2a detection. Resistance trends were compared between 2017-2020 and 2021-2024. <b>Results</b>: The leading pathogens were <i>Staphylococcus pseudintermedius</i> (ears 48.1%; skin 79.7%) and <i>Pseudomonas aeruginosa</i> (ears 29.1%; skin 7.6%). Staphylococci showed high resistance to macrolides, clindamycin, tetracycline, and first-line β-lactams (amoxicillin-clavulanate, cephalexin), with the highest susceptibilities to amikacin, florfenicol, and rifampicin. <i>P. aeruginosa</i> remained most susceptible to amikacin, polymyxin B, and imipenem. Between the two periods, <i>S. pseudintermedius</i> resistance increased to amikacin, fusidic acid, and cephalexin, while resistance to florfenicol decreased. <i>P. aeruginosa</i> resistance to imipenem increased. The prevalence of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) was 27.4% (74/270). MDR <i>S. pseudintermedius</i> and MDR <i>P. aeruginosa</i> were identified in 38.5% and 53.3% of isolates, respectively. One isolate of each species was resistant to all tested drugs. <b>Conclusions</b>: These findings confirm high levels of antimicrobial resistance in major canine skin and ear pathogens and emphasize the need for susceptibility-based therapy, rational antimicrobial use, and ongoing surveillance in small-animal practice.