Long-Term Saccharomyces cerevisiae Supplementation Enhances Milk Yield and Reproductive Performance in Lactating Dairy Cows on Smallholder Farms

This study examined the effects of long-term <i>Saccharomyces cerevisiae</i> supplementation on feed intake, milk production, milk composition, and selected reproductive indicators in lactating dairy cows. Twenty-four multiparous Holstein-Friesian crossbred cows were blocked by parity and randomly allocated to three treatments: a control group without supplementation (CON; n = 7), live yeast supplementation for 60 days (YS-60; n = 10), and live yeast supplementation for 90 days (YS-90; n = 7). Dry matter intake and body weight gain were significantly higher in cows receiving live yeast, with the greatest responses observed in the YS-90 group (<i>p</i> < 0.05). Milk yield and energy-corrected milk were increased by supplementation, particularly in YS-90 cows (<i>p</i> < 0.01), along with higher milk fat and lactose concentrations. Somatic cell count was consistently lower in YS-90 cows throughout the 14-week experimental period. Body condition score differed among treatments (<i>p</i> < 0.01), with higher values observed in yeast-supplemented cows. Feed efficiency did not differ among treatments. Reproductive parameters, including estrus detection and pregnancy rate, were not significantly affected by live yeast supplementation, although plasma progesterone concentration was higher in supplemented cows (<i>p</i> < 0.05). Given the limited number of animals per treatment, reproductive outcomes should be interpreted cautiously. Overall, extended live yeast supplementation improved production performance and udder health, while its effects on reproductive performance warrant further investigation.