Simultaneous Evaluation of Three Alternative Models to Animal Caries Testing for Assessing the Anticaries Efficacy of Monofluorophosphate Dentifrices

The in situ, in vivo, and modified in vitro models each provided dose-responsive and sensitive measures of MFP activity. Importantly, the modification of pH cycling for MFP hydrolysis enabled a well-developed laboratory protocol to be adapted to an important fluoride source. These approaches appear to offer viable alternatives to animal caries models for evaluating the efficacy of MFP-containing dentifrices and each could be chosen in different settings to understand the impact of MFP-containing dentifrices on caries prevention.