Acoustic and bioactive supplement interactions influencing rabbit growth in a bioengineered feeding system

Inconsistent feeding behaviour in rabbits remains a major challenge in livestock management, often leading to poor feeding efficiency, variable growth, and unstable environmental conditions within rearing facilities. This study focuses on the development and validation of a Controlled Feeding System (CFS) that integrates acoustic frequency modulation (AFM) and bioactive dosing using a vitamin B complex (HD) to assess its operational reliability and environmental responsiveness under pilot conditions. Twenty crossbred rabbits were distributed across nine test configurations and one control group following a 3² factorial design that combined three AFM levels (5 Hz, 10 Hz, and 20 Hz) with three HD (5 mL, 7 mL, and 10 mL). The CFS employed a microcontroller-operated MOSFET circuit to deliver auditory cues and regulate feeding intervals, thereby simulating an adaptive biosystem interface. Key performance parameters, growth rate, temperature, and relative humidity, were continuously monitored over 27 days. The combination of 10 Hz AFM and 7 mL bioactive dosing yielded the most stable synchronization between animal response and environmental control. These findings confirm the system’s functional viability and control precision, establishing the CFS as a validated model platform that integrates physiological feedback with engineered regulation. Overall, this study serves as a proof of concept demonstrating that sensory modulation and biochemical cues can be effectively coupled within a controlled framework to guide future large-scale biological applications and intelligent livestock process automation.