The Effect of Ozone on Kidney and Liver Tissues in the Treatment of Sepsis Caused by Cecal Perforation in Diabetic Mice

Hüseyin Göbüt,1 Bilgin Hasret Şimşek,2 Nurten Inan,2 Mustafa Arslan,2– 4 Kürşat Dikmen,1 Şaban Cem Sezen,5 Mustafa Kavutcu,6 Ayşegül Küçük,7 Ömer Kurtipek2 1Gazi University Faculty of Medicine, Department of General Surgery, Ankara, 06500, Turkey; 2Gazi University Faculty of Medicine, Department of Anesthesiology and Reanimation, Ankara, 06500, Turkey; 3Gazi University, Application and Research Centre for Life Sciences, Ankara, 06830, Turkey; 4Gazi University, Centre for Laboratory Animal Breeding and Experimental Research (GÜDAM), Ankara, 06500, Turkey; 5Kırıkkale University Faculty of Medicine, Department of Histology and Embryology, Kırıkkale, 71450, Turkey; 6Gazi University Faculty of Medicine, Department of Medical Biochemistry, Ankara, 06500, Turkey; 7Kutahya Health Sciences University Faculty of Medicine, Department of Physiology, Kutahya, 43020, TurkeyCorrespondence: Ayşegül Küçük, Kutahya Health Sciences University, Medical Faculty, Department of Physiology, Kutahya, Turkey, Tel +90 532 675 77 05, Email [email protected]: This study aimed to investigate the anti-inflammatory and antioxidant effects of ozone on liver and kidney tissues as an adjuvant therapy in the treatment of sepsis in individuals with diabetes.Materials and Methods: 46 Swiss Albino mice were divided into six groups: control (C), diabetes (D), diabetes + ozone (DO), diabetes + cecal perforation (DCP), diabetes + cecal perforation + ozone (DCPO), and diabetes + ozone + cecal perforation (DOCP). The diabetes groups received 125 mg/kg intraperitoneal (i.p). Streptozotocin (STZ). The DCPO and DOCP groups received 1 mL (20 μg mL−1 i.p.) ozone. Liver and kidney tissues were collected 24 hours later. Tissue samples were stored under appropriate conditions for histopathological and biochemical analyses.Results: Histopathological analysis of liver and kidney tissue revealed that all acute inflammatory markers were more pronounced in the DCP group compared to the C, D, and DO groups. Acute inflammatory markers were lower in the ozone-treated groups compared to the DCP group. In the diabetes and sepsis groups, malondialdehyde (MDA) levels increased in both liver and kidney tissues, while catalase (CAT) activity decreased. MDA levels were lower in the ozone-treated groups, and CAT activity was higher than in the no-ozonized groups. Blood urea nitrogen (BUN), creatinine, AST, and ALT levels were significantly higher in the DCP group compared to the C, D, and DO groups. Conversely, these values were lower in the DCPO and DOCP groups compared to the DCP group.Conclusion: Our findings suggest that ozone therapy may alleviate inflammatory and oxidative stress-related damage to the liver and kidneys caused by diabetes and sepsis. Additionally, ozone therapy appears to reduce serum markers of liver and kidney function such as AST, ALT, BUN, and creatinine.Keywords: diabetes mellitus, sepsis, ozone, liver, kidney