Identification of a stress-sensitive endogenous opioid-containing neuronal population in the paranigral ventral tegmental area

Nociceptin/orphanin FQ (N/OFQ), an endogenous opioid neuropeptide, and its G-protein coupled receptor NOPR have been implicated in motivation, feeding behaviors, and aversion. Stress-induced dysfunction in these states is central to the development of numerous psychiatric disorders, and the N/OFQ-NOPR system's role in reward- and stress-related responses has driven broad interest in NOPR as a therapeutic target for anxiety and depression. However, the impact of stress on N/OFQ signaling in the context of its influence on discrete midbrain reward circuitry remains unknown. To this end, we focused on a possible candidate population of N/OFQ neurons in the paranigral ventral tegmental area (pnVTA^PNOC) that have been shown to act locally on NOPR-containing VTA dopamine neurons to suppress motivation. Here we report and characterize pnVTA^PNOC sensitivity during exposure to a diverse range of stressors. Our results indicate that pnVTA^PNOC neurons become recruited during exposure to a variety of acute stressor types, suggesting that this N/OFQ population in the pnVTA could act as a critical bridge between stress and motivation.