The effect of eight weeks of swimming training, cell and laser therapy on the expression of IL-2, IL-10 and STAT-3 genes in testicular tissue in azoospermia model mice.

Background: Azoospermia means lack of sperm in semen. One percent of all men and ten percent of infertile men suffer from azoospermia, the purpose of this study is to investigate the effects of eight weeks of swimming training, cell and laser therapy on the expression of IL-2, IL-10 and STAT-3 genes in testicular tissue. It is azoospermia in rats. Methods: Forty 6- to 8-week-old rats were randomly selected, and then the azoospermia model was induced with Busulfan at a dose of 40 mg in mice. One month after induction of the model, stem cells were transplanted once in the vas deferens at the rate of one million cells per mouse. Then, one week after cell transplantation, a laser with a wavelength of 632.8 nm and a power of 10 mW and energy 3 joules were applied in three repetitions throughout the study period with an interval of once a week, and after improving the effects of surgery, swimming training were performed for 30 minutes and 5 days a week for 8 weeks. Results: The results showed that induction of azoospermia model caused a significant increase in IL-2, IL-10 and STAT-3 genes compared to healthy group rats (P≤0.05). Also, performing the interventional methods of laser therapy, cell therapy and exercise significantly reduced the expression of these genes in the testicular tissue of rats compared to the patient group (P≤0.05). Conclusion: Swimming exercise in combination with cell and laser therapy may help the fertility of azoospermia model rats by reducing testicular tissue inflammation signaling.