Prospective Evaluation of Specific IgE Profile and Quality-of-Life During Allergen-Specific Immunotherapy with House Dust Mite: A Pilot Study

<i>Background and Objectives</i>: The average prevalence of sensitization to house dust mite in developed countries is more than 20%. The three major allergens of <i>D. pteronyssinus</i>-Der p 1, Der p 2, and Der p 23-have been associated with asthma severity. Allergen-specific immunotherapy (ASIT) is the only personalized and effective treatment that can change the natural course of allergic diseases such as allergic rhinitis or allergic asthma. Despite ASIT being an established treatment method, its effectiveness is still assessed using patient-reported outcome measures that determine quality of life, and there are no objective biomarkers that can accurately and reliably indicate the therapeutic efficacy of ASIT. This study aimed to monitor sensitization profiles to allergens, assess the effectiveness of ASIT, and evaluate total nasal symptom score (TNSS) and quality of life after six months of ASIT treatment. <i>Materials and Methods</i>: The molecular allergy diagnostic system was used to assess changes in patients' sensitization profiles to allergens, and the validated questionnaires RQLQ and TNSS were used for quality-of-life assessment. <i>Results</i>: After 6 months of ASIT treatment against house dust mite allergens, a statistically significant increase in sIgE against the Der p 23 component was noted. In addition, a significant decrease in practical problems and an improvement in patients' emotional state were observed, while the TNSS score remained unchanged. <i>Conclusions</i>: Continuous monitoring of the Der p 23 component during further stages of ASIT is, therefore, essential to determine whether the observed changes reflect de novo sensitization or represent an immunological response to therapy.