An Nlrp5-null mutation leads to attenuated de novo methylation in oocytes, accompanied by a significant reduction in DNMT3L.

Acknowledgements: We would like to acknowledge the invaluable support of the Babraham BSU for animal maintenance and husbandry, and the excellent guidance and assistance from the Babraham Proteomics, Imaging, Sequencing, and Bioinformatics facilities, with special thanks to Judith Webster, Simon Walker, and Megan Hamilton. We would also like to acknowledge Simon Andrews and Sarah Inglesfield from the Babraham Bioinformatics facility for initial sequencing data processing and QC, and Jurrien Dean and Shoji Tajima for providing MATER (NALP5) and DNMT3L antibodies, respectively.