Effect of Dispersed Particle Concentration on Photoacoustic Flowmetry Using Low-Frequency Transducers

Photoacoustic (PA) velocimetry offers a promising solution to the limitations of conventional techniques for measuring blood flow velocity. Given its moderate penetration depth and high spatial resolution, PA imaging is considered suitable for measuring low-velocity blood flow in capillaries located at moderate depths. High-resolution measurements based on PA signals from individual blood cells can be achieved using a high-frequency transducer. However, high-frequency signals attenuate rapidly within biological tissue, restricting the measurable depth. Consequently, low-frequency transducers are required for deeper measurements. To date, PA flow velocimetry employing low-frequency transducers remains insufficiently explored. In this study, we investigated the effect of the concentration of particles that mimic blood cells within vessels under low-concentration conditions. The performance of flow velocity measurement was evaluated using an ultrasonic transducer (UST) with a center frequency of 10 MHz. The volume fraction of particles in the solution was systematically varied, and the spatially averaged flow velocity was assessed using two different distinct analysis methods. One method employed a time-shift approach based on cross-correlation analysis. Flow velocity was estimated from PA signal redpairs generated by particles dispersed in the fluid, using consecutive pulsed laser irradiations at fixed time intervals. The other method employed a pulsed Doppler method in the frequency domain, widely applied in ultrasound Doppler measurements. In this method, flow velocity redwas estimated from the Doppler-shifted frequency between the transmitted and received signals of the UST. For the initial analysis, numerical simulations were performed, followed by experiments based on displacement measurements equivalent to velocity measurements. The target was a capillary tube filled with an aqueous solution containing particles at different concentration levels. The time–domain method tended to underestimate flow velocity as particle concentration increased, whereas the pulsed Doppler method yielded estimates consistent with theoretical values, demonstrating its potential for measurements at high concentrations.