Mitochondrial function and reactive oxygen species dynamics in Italian Mediterranean buffalo semen following cryopreservation and post-thaw incubation

In conclusion, cryopreservation and subsequent incubation significantly affect mitochondrial functions, which strongly correlate with sperm motility. A deeper understanding of sperm energy metabolism and its relationship with redox regulation could allow for the optimization of current assisted reproductive technologies (ART), as these factors play a crucial role in sperm viability, motility, and fertilization capacity, which are critical for optimizing outcomes in ART procedures.