Structural and Anti‐Inflammatory Activity of Ultrasonic Treating Polysaccharide Extracted From <i>Tremella fuciformis</i> Stem Byproducts

ABSTRACT This study investigated the structural characteristics and anti‐inflammatory potential of Tremella fuciformis stem byproduct‐derived polysaccharide TFP‐1. Structural analysis revealed that TFP‐1 is an acetylated heteropolysaccharide composed of mannose, xylose, fucose, and glucuronic acid in a molar ratio of 6.18:1.09:2.13:1. It likely has a 3‐α‐ d ‐Man p backbone, branched by T‐α‐ l ‐Fuc p , 2‐β‐ d ‐Xyl p , and T‐β‐ d ‐GlcA p at the O‐2 position, with partial acetylation at C6‐OH of mannoses. The anti‐inflammatory activity of TFP‐1 was also assessed. It was shown to inhibit the production of inflammatory factors in lipopolysaccharide (LPS)‐stimulated RAW264.7 macrophages by regulating the NF‐κB pathway. These findings suggest TFP‐1 may serve as a potential anti‐inflammatory supplement.