Demystifying AAV affinity capture: mapping AAV-affinity ligand footprints with cryo-electron microscopy

Efficient and cost-effective purification is essential for achieving key objectives in AAV production including maximizing yield, maintaining product quality, and streamlining downstream processes. This poster highlights a study that leverages cryo-electron microscopy (cryo-EM) to map precise interactions between affinity ligands and AAV capsids. Combining robust resin performances—capable of 20+ clean-in-place cycles—with detailed molecular mapping optimizes capsid-resin compatibility, thereby reducing risks, saving time, and ensuring scalable, cost-effective downstream workflows.