Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection of Listeria monocytogenes in food samples

The developed sandwich ELISA provides a sensitive, specific, rapid, and regionally tailored diagnostic platform for detecting pathogenic <i>L. monocytogenes</i> in food samples. By integrating locally produced recombinant antigens and immunoreagents, the assay offers a cost-effective alternative to imported kits, supporting national food safety programs and One Health surveillance initiatives.