Comprehensive SSR study of 14 Zingiberaceae species based on microsatellite capture sequencing (MiCAPs)

This integrated workflow combining MiCAPs, ePCR, and PSR demonstrates a practical approach for marker development and diversity assessment in polyploid species lacking reference genomes. Despite the genetic complexities inherent in Zingiberaceae, especially potential polyploidy, our approach proved highly effective in establishing a robust phylogenetic framework and enabling comprehensive genetic diversity assessment. The novel set of 612 SSR marker candidates represents a significant resource that will facilitate future genetic studies focused on the diversity, evolutionary relationships, conservation, and sustainable utilization of valuable Zingiberaceae species.