ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION AND PERCENTAGE RECOVERY OF NATURAL ACTIVE CONSTITUENTS (CURCUMIN AND PIPERINE) IN MARKETED PREPARATION BY UV METHOD

This study establishes a validated UV spectrophotometric method for the simultaneous estimation of Curcumin and Piperine, supported by extensive pre-formulation characterization. Organoleptic properties, solubility profiling, pH measurement, melting point analysis, and FTIR spectroscopy confirmed the identity, purity, and stability of both compounds. UV analysis identified λmax values of 424 nm for Curcumin and 345 nm for Piperine, with an overlay peak at 367 nm suitable for simultaneous detection. The method demonstrated strong linearity (R² > 0.99), good sensitivity with low LOD and LOQ values, and acceptable precision in intra-day, inter-day, and repeatability studies (%RSD < 2%). Recovery values (Curcumin: 96.0%, Piperine: 98.10%) confirmed method accuracy. Although high %RSD in certain calibration and robustness evaluations suggests a need for better control of sample preparation and temperature, the method remains simple, reliable, and suitable for routine quality control of herbal and pharmaceutical formulations. Overall, the study provides a robust analytical foundation for future formulation development involving Curcumin and Piperine.