ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ALOIN AND THIOCOLCHICOSIDE BY UV-VISIBLE SPECTROPHOTOMETRY

The present study aimed to develop and validate a simple, accurate, precise, and cost-effective UV–Visible spectrophotometric method for the quantitative estimation of Aloin and Thiocolchicoside, two pharmacologically active anti-inflammatory agents. Both drugs were analyzed individually using methanol as a solvent after determining their maximum absorption wavelengths (λmax) at 295 nm for Aloin and 366 nm for Thiocolchicoside. For simultaneous estimation, a common analytical wavelength of 305 nm was selected. The developed method was validated in accordance with ICH Q2(R1) guidelines by evaluating parameters such as linearity, accuracy, precision, specificity, ruggedness, robustness, and sensitivity. Linearity was established within the concentration range of 10–60 µg/mL for Aloin and 5–30 µg/mL for Thiocolchicoside, with correlation coefficients (R²) of 0.9999 and 0.9992, respectively, indicating excellent linear relationships. The %RSD values for intra-day, inter-day, and repeatability studies were all below 2%, confirming method precision. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 2.01 µg/mL and 7.09 µg/mL for Aloin, and 1.95 µg/mL and 6.10 µg/mL for Thiocolchicoside, respectively, indicating high method sensitivity. Recovery studies demonstrated accuracy within the acceptable range of 98–102%. The developed UV spectrophotometric method proved to be rapid, reliable, and economical, making it suitable for routine quality control analysis and quantitative determination of Aloin and Thiocolchicoside in bulk and pharmaceutical dosage forms.