Southern Chilean Native Plants as Novel Sources of Antioxidant and Antibacterial Extracts

The temperate rainforests of southern Chile host a rich diversity of plants traditionally used in medicine, yet their bioactive potential remains underexplored. This study evaluated the chemical composition, antioxidant capacity, antibacterial activity, and cell viability of ethanolic leaf extracts from <i>Cissus striata</i> (CS), <i>Mitraria coccinea</i> (MC), and <i>Raukaua laetevirens</i> (RL), compared with <i>Buddleja globosa</i> (BG), a well-known medicinal shrub. Extracts were obtained using 70% ethanol, ensuring high recovery of polyphenolic compounds while avoiding thermal degradation. The total phenolic content (TPC) was highest in CS, exceeding values reported for green tea, while MC exhibited the greatest total flavonoid content (TFC). HPLC-MS/MS analysis showed that RL was rich in rutin, while CS exhibited a higher quercetin content. Antioxidant activity assessed through ABTS, DPPH, and FRAP assays, was correlated with polyphenolic composition. CS showed the highest antioxidant potential, surpassing green tea by ~39%, as determined via FRAP, while MC and RL displayed capacities comparable to BG. Antibacterial activity assays demonstrated that MC inhibited <i>Escherichia coli</i> with a minimum inhibitory concentration (MIC) of 12.5 mg/mL, lower than that of ampicillin, whereas CS was highly active against <i>Staphylococcus aureus</i>, with an MIC of 0.39 mg/mL, equivalent to the activity exhibited by tetracycline. Cytotoxicity assays confirmed that the extracts did not reduce human cell viability, supporting the potential of Chilean native shrubs as safe, natural sources of antioxidants and antimicrobials for food and pharmaceutical applications.