Deep phenotypic and cytotoxicity characterization of NK cells cultured with chemically defined additives

Immunotherapy is transforming cancer treatment by harnessing the body’s immune system, with natural killer (NK) cells emerging as promising alternatives to T cells due to their innate cytotoxicity. However, delivering effective NK cell therapies highly depends on the ancillary materials used during ex vivo bioprocessing and robust cell characterization. This poster outlines a workflow from NK cell isolation to serum-free culture and cytotoxic analysis, offering a comprehensive view of NK cell function through targeted killing, surface marker profiling, and secreted molecule characterization.