Integrated multi-omics unveil the impact of H-phosphinic analogs of glutamate and α-ketoglutarate on Escherichia coli metabolism

Funding Information: FG is grateful to Sapienza University of Rome for a scholarship specifically devoted to training abroad, to the Federation of European Microbiology Societies (FEMS) for grant FEMS-GO-2020 to 208 to carry out his work at ITQB NOVA (Oeiras, Portugal), and to the COST Action CA18113 EuroMicropH (https://euromicroph.eu/stsm/) for a Short Term Scientific Mission grant to visit DDB lab. LGG was financed by an FCT contract according to DL57/2016, [SFRH/BPD/111100/2015]. A. K. A. V. C. D. D. B. J. A. and L. G. G. writing\u2013review & editing; A. K. J. A. and L. G. G. methodology; A. K. A. V. C. and D. D. B. funding acquisition; A. V. C. J. A. and L. G. G. validation; A. V. C. and D. D. B. supervision; A. V. C. D. D. B. A. K. and J. A. resources; A. V. C. D. D. B. and F. G. conceptualization; D. D. B. F. G. and L. G. G. visualization; D. D. B. project administration; F. G. writing\u2013original draft; F. G. investigation; F. G. J. A. and L. G. G. formal analysis; J. A. and L. G. G. data curation. This research was funded by Sapienza University of Rome (Progetti Medi di Ateneo n. RM11916B861B9985 and RM120172B6587496) and by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia, Portugal I.P. through MOSTMICRO-ITQB R&D Unit (DOI 10.54499/UIDB/04612/2020; DOI 10.54499/UIDP/04612/2020) and LS4FUTURE Associated Laboratory (DOI 10.54499/LA/P/0087/2020). NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT (project AAC 01/SAICT/2016). The metabolomic data deposit was by NIH grant U2C-DK119886 and OT2-OD030544 grants. Syntheses of L-Glu-\u03B3-PH, rac-Glu-\u03B3-PH, and \u03B1-KG-\u03B3-PH were supported by Russian Science Foundation (grant No 22-14-00291). The authors are grateful to PNNL and the Venn-Diagram-Plotter GitHub repository for Venn Diagram Plotter. F. G. is grateful to Sapienza University of Rome for a scholarship specifically devoted to training abroad, to the Federation of European Microbiology Societies (FEMS) for grant FEMS-GO-2020-208, and to the COST Action CA18113 EuroMicropH (https://euromicroph.eu/stsm/) for a Short Term Scientific Mission grant to visit D. D. B. lab. L. G. G. was financed by an FCT contract according to DL57/2016, [SFRH/BPD/111100/2015]. Funding Information: FG is grateful to Sapienza University of Rome for a scholarship specifically devoted to training abroad, to the Federation of European Microbiology Societies (FEMS) for grant FEMS-GO-2020-208 to carry out his work at ITQB NOVA (Oeiras, Portugal), and to the COST Action CA18113 EuroMicropH ( https://euromicroph.eu/stsm/ ) for a Short Term Scientific Mission grant to visit DDB lab. LGG was financed by an FCT contract according to DL57/2016, [SFRH/BPD/111100/2015]. Funding Information: This research was funded by Sapienza University of Rome (Progetti Medi di Ateneo n. RM11916B861B9985 and RM120172B6587496) and by FCT - Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia, I.P., through MOSTMICRO-ITQB R&D Unit (DOI 10.54499/UIDB/04612/2020; DOI 10.54499/UIDP/04612/2020) and LS4FUTURE Associated Laboratory (DOI 10.54499/LA/P/0087/2020). NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT (project AAC 01/SAICT/2016). The metabolomic data deposit was by NIH grant U2C-DK119886 and OT2-OD030544 grants. Syntheses of L-Glu-\u03B3-P H , rac-Glu-\u03B3-P H , and \u03B1-KG-\u03B3-P H were supported by Russian Science Foundation (grant No 22-14-00291). The authors are grateful to PNNL and the Venn-Diagram-Plotter GitHub repository for Venn Diagram Plotter. Publisher Copyright: © 2024 The Authors