Backbone Modification with β-Amino Acids Alters the Stability and Immunogenicity of MUC1-Derived Glycopeptide Formulations

Funding Information: This project has received partial funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 956544. This work was also supported in part by the National Institutes of Health grant (R01 GM056414 and its successor R35 GM151985) awarded to S.H.G. We thank the Agencia Estatal de Investigación (AEI, PID2021-127622OB-I00 to F.C., J.H.B. and O.S., PDC2022-133725-C21 to F.C, J.H.B., PID2020-120099RA-I00 to F.G.M., PID2021-124328OB-100 to J.A., and PRE-2019-091720 to S.A.), Universidad de La Rioja (REGI22/47 and REGI22/16) and Asociación Española contra el Cáncer (AECC) sección La Rioja (F.C. and J.M.P.). F.M. and A.S.G. acknowledge Fundação para a Ciência e Tecnologia Portugal (FCT-Portugal) for the MGL4Life project 10.54499/PTDC/QUI-OUT/2586/2020. F.M. thanks FCT-Portugal for CEECINST/00042/2021/CP1773/CT0011. F.M and A.S.G. thank UCIBIO project (UIDP/04378/2020 and UIDB/04378/2020), and Associate Laboratory Institute for Health and Bioeconomy - i4HB project (LA/P/0140/2020) and the National NMR Facility supported by FCT-Portugal (ROTEIRO/0031/2013–PINFRA/22161/2016, cofinanced by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC). T.S. thanks MCIN/AEI/10.13039/501100011033 and the European Union NextGenerationEU/PRTR for his Juan de la Cierva contract, JDC2022-048607-I. N.G. held a predoctoral grant from the Scientific Foundation of the Spanish Association Against Cancer in Bizkaia (PRDVZ222452GUTI). CICbioGUNE is the recipient of a Severo Ochoa Centro de Excelencia Award (CEX2021-001136-S). Supported in part by Fundación Jesús de Gangoiti. We are also grateful for partial support from the Vilas Trust and from the University of Wisconsin-Madison Office of the Vice Chancellor for Research and Graduate Education with funding from the Wisconsin Alumni Research Foundation. A.J.K. was supported in part by a postdoctoral fellowship from the National Institutes of Health (5F32AI176876). Publisher Copyright: © 2025 The Authors. Published by American Chemical Society.