A long-lasting porcine model of ARDS caused by Pseudomonas aeruginosa and Ventilator-Induced Lung Injury

[eng] INTRODUCTION: Existing animal models of acute respiratory distress syndrome (ARDS) are varied and often differ compared to human ARDS in their main features of lung damage, etiology, pathophysiology, or maintenance over time. In this thesis, we characterize a new model of this disease induced by pulmonary sepsis due to Pseudomonas aeruginosa and mechanical ventilation-induced lung injury (VILI) that precisely resembles human ARDS. HYPOTHESIS: The porcine model of ARDS developed in this thesis by means of P. aeruginosa and lung damage induced by mechanical ventilation, is capable of accurately reproducing the human disease. These characteristics include the Berlin criteria, as well as being a reliable model, being maintained in the long term and being irreversible for the duration of the experiments. In addition, as a result of the expected severe alteration, the penetration of the antibiotic into the tissue will be modified. OBJECTIVES: The objectives are mainly to be able to induce ARDS with the combination of a P. aeruginosa inoculum and harmful mechanical ventilation, studying the variables of the process; and to analyze the pharmacokinetics of intravenous meropenem as a treatment of pneumonia.