GC-MS PROFILE AND INVITRO ANTI-DIABETIC, ANTI-INFLAMMATORY, AND ANTIOXIDANT POTENTIALS OF THE AQUEOUS LEAF EXTRACT OF Chromolaena odorata

Chromolaena odorata belongs to the family Asteraceae which is traditionally used as antidiarrheal, astringent, anti-inflammatory, wound healing, anti-hypertensive, and anti-pyretic. The aim of this study was to determine the anti-diabetic, anti-inflammatory, and antioxidant potentials of the aqueous leaf extract C. odorata. The phytoconstituents were assayed using GC-MS analysis. The In-vitro anti-diabetic activity (alpha amylase inhibitory activity) was assayed, anti-inflammatory activity (albumin denaturation, trypsin inhibitory activity and lipoxygenase activity) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Potential (FRAP), Reducing power and ABTS assays were carried out using standard procedure. The result revealed that the aqueous leaf extract of Chromolaena odorata had an anti-diabetic potential by inhibiting alpha amylase activity when compared to the standard (Acarbose). The aqueous leaf extract of Chromolaena odorata had a significant (p<0.05) increased anti-inflammatory activity by inhibiting albumin denaturation, trypsin activity, and lipoxygenase activity. C. odorata aqueous leaf extract had a significantly lowered IC50 for ABTS (0.057mg/ml) and DPPH (0.073mg/ml) scavenging activity compared to the standard (Ascorbic acid). In conclusion, the aqueous leaf extract of C. odorata exhibited anti-diabetic, anti-inflammatory, and antioxidant activity which could be useful in various fields such as in healthcare, industrial purposes, food. KEYWORDS: Chromolaena odorata, Diabetes, Inflammation, Antioxidant, GCMS DOI: 10.7176/JNSR/16-2-07 Publication date: November 30th 2025