Exploring Replicate Inconsistencies and Bioinformatic Tool Disparities

Funding Information: We acknowledge support provided by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia, Portugal through projects AgriWWAter (PTDC/CTA AMB/29586/2017), VirusFreeWater (706, Internalproject IBETXplore 2017) and Internal exploratory Project WasteWaterVir (GHTM-UID/04413/2020), through funds to GHTM\u2014UID/04413/2020 and LA-REAL\u2014LA/P/0117/2020, and through contrato-programa 1567 (https://doi.org/10.54499/CEECINST/00102/2018/CP1567/CT0040 attributed to S.G.S. Support was also provided by Project 101186531\u2014DxHub, funded by the European Union. Views and opinions expressed are, however, those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Executive Agency (REA). Neither the European Union nor the granting authority can be held responsible for them. MTBC acknowledges support of the Research Unit UID/04462: iNOVA4Health\u2014Programme in Translational Medicine, financially supported by FCT/Minist\u00E9rio da Educa\u00E7\u00E3o, Ci\u00EAncia e Inova\u00E7\u00E3o and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). Publisher Copyright: © 2025 by the authors.