Phylogenetic Analysis of Freshwater Fish (Cyprinidae And Danionidae) from Diatas and Dibawah Lakes using COI Gene

Genetic information in an ecosystem is crucial for determining effective population management, conservation, and breeding strategies for freshwater fish in various waters, including Diatas and Dibawah Lakes, West Sumatra. Phylogenetic analysis of freshwater fish in Diatas and Dibawah lakes is needed to provide genetic data and information. Molecular analysis of the 33 DNA sequences (COI gene) was carried out on the dominant fish groups (Cyprinidae and Danionidae) at several sites in Diatas and Dibawah Lake. Phylogenetic analysis revealed a monophyletic relationship between the Cyprinidae and Danionidae in Diatas and Dibawah lakes. The Cyprinidae group has a genetic distance of 20.2% to 21.3% with Danionidae. The genetic distance among members of Cyprinidae is 13.6% to 20.4%, and 10.3% among members of Danionidae. Neolissochilus sumatranus and Tor douronensis have the lowest genetic distance (1.0%), and Barbodes banksi and Rasbora lateristriata have the highest genetic distance (24.7%). The genetic distance between intra and inter-populations for each species is 0.0%, respectively. Members of each species are represented by a single haplotype, respectively. Genetic information indicates that all populations are derived from a single stock unit.