Metal-specific differences of the exometabolites produced by serpentinite-associated

The endolithic bacteria found in serpentinite rocks are a promising resource for biotechnological application. The present study investigates the metal-specific response of exometabolites produced by the serpentinite-isolated strain Pseudomonas chlororaphis S15. The integration of HPLC metabolomic profiling with CAS assay analysis has been demonstrated to reveal that the strain employs highly specific adaptive strategies. While both Co2+ and Ni2+ suppressed the core exometabolome, Co2+ was found to uniquely induce the de novo synthesis of a novel compound. In contrast, Fe3+ and Ga3+ were found to stimulate metabolite production and siderophore activity. These findings reveal metal-specific metabolic plasticity in P. chlororaphis S15, which has significant potential for the development of targeted environmental biotechnologies.