Optimal Extraction of Monoammonium Glycyrrhizate from Licorice Root (Glycyrrhiza Glabra) by Hydrothermal Technology

A novel methodology has been established, enhancing the effectiveness of extraction methods for monoammonium glycyrrhizate derived from licorice root powder through hydrothermal technology, utilizing the Response Surface Methodology (RSM). By varying extraction solvents, techniques, durations (ranging from 15 to 45 minutes), and temperatures (between 150 and 200 °C), optimal extraction conditions were identified. The study measured 47.15 mg/g of monoammonium glycyrrhizate, while also assessing the impact of operating pressure (from 0.5 to 2.5 MPa) on the response variables of monoammonium glycyrrhizate using High-Performance Liquid Chromatography (HPLC). The findings revealed that, at a fixed operating pressure, a significant increase in extraction temperature corresponded with an enhanced extraction rate of monoammonium glycyrrhizate from licorice root. Additionally, the combined influence of extraction temperature and duration, as well as operating pressure and time, exhibited a saddle curve pattern, indicating that the maximum extraction rate for these bioactive compounds occurred at 30 minutes. The optimal parameters for extracting bioactive compounds via hydrothermal technology were determined to be 200 °C, 30.29 minutes, and 0.5 MPa, yielding a desirability score of 0.75, with monoammonium glycyrrhizate concentrations measured at 47.15 mg/g, respectively. This investigation underscores the potential of hydrothermal technology and environmentally benign Methods for the effective extraction of bioactive compounds from licorice root, presenting significant opportunities for Utilization in the food sector, sectors of medicine, and pharmaceutical.