plant flower and leaf growing in Uzbekistan

This study investigates the antiradical activity of Inula helenium L. (elecampane) leaves and flowers using the 2.2-diphenyl-1- picrylhydrazyl (DPPH) assay. The HPLC-UV method was employed to monitor the discoloration of the purple DPPH solution, which indicates hydrogen atom donation or electron transfer by antioxidant compounds. Samples were prepared at concentrations of 50, 100, 150, and 200 μL by mixing plant material with 3 mL of DPPH solution. Absorbance was measured at 517 nm wavelength to determine the concentration required for 50% inhibition (IC50) of radical activity. Measurements were recorded after 10 minutes of reaction time across all tested concentrations. Results demonstrated significant antiradical activity in Inula helenium L., with an IC50 value of 645.2 μL. These findings confirm the potent radical- scavenging capacity of elecampane leaves and flowers, suggesting their potential value in preventive healthcare and therapeutic applications for oxidative stress-related disorders.