AN179 antibody recognizes specifically L1CAM by flow cytometry on rhabdomyosarcoma cell lines

L1 cell adhesion molecule (L1CAM) is a relevant target in pediatric tumors, including rhabdomyosarcoma (RMS). We validated the recombinant antibody ABCD_AN179, derived from the CE7 hybridoma, for specific detection of L1CAM by flow cytometry. Using CRISPR/Cas9-engineered L1CAM-knockout RMS cell lines (RD, Rh30), ABCD_AN179 showed clear staining of wild-type cells but no signal in knockouts, confirming high specificity. These results demonstrate that ABCD_AN179 specifically detects human L1CAM by flow cytometry and represents a validated recombinant antibody that supports research and translational studies.