Smart illumination for 3D-imaging of biological tissues

Three dimensional imaging of living tissues with widefield fluorescence optical microscopy is limited by out-of-focus blur, light dose, and acquisition speed. We discuss recent camera-based strategies that use light more intelligently to improve resolution, sectioning and reduce toxicity when imaging thick biological structures.

Publication Details

Journal
Springer Link (Chiba Institute of Technology)
Published
2025-11-28
DOI
https://doi.org/10.1051/photon/202513436/pdf
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
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article

Smart illumination for 3D-imaging of biological tissues

Springer Link (Chiba Institute of Technology)
Advanced Fluorescence Microscopy Techniques
article

Smart illumination for 3D-imaging of biological tissues

article en

Abstract

Three dimensional imaging of living tissues with widefield fluorescence optical microscopy is limited by out-of-focus blur, light dose, and acquisition speed. We discuss recent camera-based strategies that use light more intelligently to improve resolution, sectioning and reduce toxicity when imaging thick biological structures.

Springer Link (Chiba Institute of Technology)
Openalex Percentile: Top 25%
Advanced Fluorescence Microscopy Techniques
DOISourceOpenAlex
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Smart illumination for 3D-imaging of biological tissues · Springer Link (Chiba Institute of Technology) (2025) | TGRS Research Map | TGRS