Anisomycin recapitulates CHX behavior when combined with HU or DIA [Dataset]

(A) Quantification of the incidence of the N-Cap phenotype in a wild-type strain during 3 mM DIA treatment (upper graph) or 75 mM HU treatment (lower graph) when combined with 100 μg/mL anisomycin (ANM). Graphs show the mean ± SD of two independent repetitions of the experiment, and in each repetition at least 100 cells were accounted for each condition. (B) Confocal microscopy images of cells expressing Hsp104-GFP (green) and mCherry-AHDL (magenta) after a 3-hour incubation in either control conditions, 100 μg/mL ANM, 3 mM DIA, 75 mM HU, DIA and ANM, or HU and ANM. Hsp104-GFP aggregates are detected only in DIA and HU treatments. Images are SUM projections of 3 central Z slices. Scale bars represent 5 μm. Source data for this figure can be found in S1 Data.