<i>Poglut1-cKO</i> muscles do not show abnormal grip strength when normalized by body weight but exhibit reduced quantal content.

<b>A)</b> Grip test of control and <i>cKO</i> mice normalized by body weight. <b>B)</b> Electrophysiological recordings from LAL muscles at low frequency stimulation (0.5 Hz, 100 s). <b>C)</b> Quantification of the EPP amplitude, miniature EPP (mEPP) amplitude, and the quantal content from control and <i>Poglut1-cKO</i> muscles. The mean mEPPs amplitude showed no significant difference between control and cKO fibers, suggesting comparable spontaneous release. While the mean size of the EPPs showed no significant difference between cKO and control, the quantal content values of cKO were significantly lower than that in control, suggesting an alteration in neurotransmission in cKO mice (<i>Poglut1-Ctr</i>: 3 mice (52 terminals); <i>Poglut1-cKO</i>: 3 mice (33 terminals)). In A and C, each dot represents an animal. Mean±SD is shown. Two-way ANOVA with Šidák’s multiple comparisons test (A); Unpaired <i>t</i> test (C). Row factor (the overall effect of genotype) is shown for A. ns: not significant, *<i>P</i> < 0.05. (TIF)