Investigation of moisture binding forms of metabiotic food systems

Microbiological production of polysaccharides has undeniable advantages over traditional methods, since it provides an environmentally friendly process for producing functional food. In order to develop methods for modulating and optimizing the synthesis of moisture-binding metabolites of polysaccharide nature, a study of the activity of exopolysaccharide production by a consortium of probiotic microorganisms (Streptococcus thermophiles, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus fermentum. Lactobacillus plantarum) under various thermal exposure conditions. An analysis of the effect of temperature inactivation modes (higher and lower values) was carried out, as well as an analysis of the effect of the duration of inactivation of probiotics. The initial concentrations of microorganisms in the probiotic consortium were at least 109 CFU/ml. On the basis of experimental data, a temperature range from 92 to 120 °C and its corresponding endothermic effects were established. The obtained data make it possible to state the presence of additional binding of moisture by microbial polysaccharides. At the end of the thermal inactivation process, the weight of the test samples was 13,65 % and 14,84 %, of the control sample - 12.36 %. The mass fraction of moisture removed in the test samples was 9,48 % and 11,39 %, in the control sample – 6,92 %, which confirms the activation of the production of microbial metabolites. The maximum activity of the synthesis of moisture-binding metabolites was noted with the following parameters of inactivation modes: temperature 75-2 °C; hold for 50 min followed by cooling to 4-2 оС.