New insights in cell cycle and meiosis regulation in fission yeast

This thesis is divided into three different sections. The first two sections involve the study of cell cycle and the third section is focused on meiosis. In the first chapter, we have developed a fluorescent-localized cell cycle reporter strain (FLCCR) that enables cell cycle monitoring in life-cell microscopy and have applied it to the study of the role of the MAPK Sty1 in cell cycle. In the second chapter we show how the activity of the G1/S transcription factor MBF is regulated in a cell cycle-dependent manner by the binding and release of the co-repressors Nrm1/Yox1 and driven by CDK phosphorylation and proteasome degradation of Nrm1. Finally, in the third chapter we present data at transcriptomic, proteomic and phosphorylation landscape levels in the regulation of meiosis initiation and progression in fission yeast. Here, we focused on the regulation of protein stability by phosphorylation and identified potential candidates which protein levels may be regulated by phosphorylation.