A Comparative Study of Direct LDL-Cholesterol Level by Different Laboratory Methods in Patients with Diabetes and Diabetic Complications

Background:The accuracy of low-density lipoprotein-cholesterol (LDL-C) calculation is critical because of the significant role that LDL-C concentration plays in identifying diabetes individuals with heart complications.Formulas of many kinds have been introduced.The accuracy of several formulas for estimating LDL-C was assessed in this study.To compare the LDL-C measured by direct homogeneous assay over a wide range of serum TG, serum TC, serum HDL-C, and serum VLDL levels in diabetic patients with heart complications with the LDL-C calculated using 12 formulas.Materials and methods: Patients who visited the central laboratory of biochemistry provided samples for lipid profile analysis.The consent of the participants was obtained before this, out of 60 samples examined in total.Individuals having a clinical history of metabolic disorders (chronic renal failure, diabetes) and who had fasted for at least 10 hours the night before were included.Excluded samples had triglyceride concentrations more than 400 mg/dL.Results: Patients with TG > 400 mg/dL were excluded from subsequent analysis.We analyzed serum LDL by the direct method in diabetic patients with cardiac complications and compared this result with different available calculated methods of LDL.The LDL calculated by Friedewald formula was 96.85 38.24 mg/dL and by the direct method was 94.88 34.62 mg/dL.The correlation coefficient between direct LDL and Friedewald formula was 0.958 and the highest correlation was seen as the most appropriate/clinically correlated.Conclusion: This result suggests that in comparison to other methods, the Friedewald may be an improved option for measuring LDL-C.